Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual |
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Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual Alison Adams No preview available - 1998 |
Common terms and phrases
acetate acid actin ade2 allele analysis auxotrophic buffer cells centrifuge chromosome Cold Spring Harbor colonies complementation concentration containing containing 5 ml crosses culture tubes cytoplasmic determine dilution diploid EXPERIMENT expression filter formaldehyde four frequency fusion gene genetic glass glucose GROUP grow growth haploid hybrid Incubate at 30°C Inoculate integration isolated leu2 mapping markers master MATa mating medium method mg/ml microfuge minutes mitotic ml of YPD mutants Na2 EDTA Note overnight patch pellet phenotype Place plasmid plates Sterile velveteen Prepare PROCEDURE protein recombination replacement Replica plate Resuspend room temperature Saccharomyces cerevisiae Safety Score selection separate single solution spores sporulation step sterile Sterile velveteen pads stock solution Store strains streak Techniques and Protocols transfer transformants Tris-Cl pH trpl ura plates URA3 vector volume Wash wild-type yeast YPD plate zygotes
Bibliographic information
| Title | Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual |
| Authors | Chris Kaiser, Susan Michaelis, Aaron Mitchell |
| Editor | Cold Spring Harbor Laboratory |
| Edition | illustrated |
| Publisher | Cold Spring Harbor Laboratory Press, 1994 |
| Original from | the University of Wisconsin - Madison |
| Digitized | Jul 13, 2010 |
| ISBN | 0879694513, 9780879694517 |
| Length | 234 pages |
| Subjects | › Science / General |
| Export Citation | BiBTeX EndNote RefMan |