Carbohydrate Biotechnology Protocols

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Springer Science & Business Media, Jan 1, 1999 - Science - 337 pages
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In Carbohydrate Biotechnology Protocols, Christopher Bucke has brought together a compilation of modern hands-on methods for the effective use of microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation biotechnologists, and applied enzymologists cutting-edge techniques-many of them hitherto unavailable in print-that are cleaner and often less costly than available chemical alternatives. Timely and readily reproducible, these state-of-the-art protocols allow the user to produce and.
 

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Contents

Introduction to Carbohydrate Biotechnology
1
OnePot Enzymatic Synthesis of Sialyl fEpitope
14
Alginate from Azotobacter vinelandii
23
Production of Schizophyllan
43
Enzymatic Synthesis of Cellulose
57
Modification of Alginate Using Mannuronan C5Epimerases
71
Viscosity Control of Guar Polysaccharide Solutions
79
The Production of Cyclodextrins Using CGTase
89
Hydrolysis of Hemicelluloses Using Combinations of Xylanases
183
Enzymatic Depolymerization of Chitins and Chitosans
197
Synthesis of Homo and HeteroOligosaccharides
213
Use of FluorophoreAssisted Carbohydrate Electrophoresis
221
Application of Sucrose Synthase in the Synthesis
235
Production of Isomaltulose Using Immobilized Bacterial Cells
255
The Production of Mannitol by Fermentation
261
The Production of 3KetoDerivatives of Disaccharides
277

Production of Microbial Glycolipids
103
Partial Enzymatic Hydrolysis of Starch to Maltodextrins
119
The Production of a1 2Terminated Glucooligosaccharides
129
Enzymatic Production of Fructooligosaccharides from Sucrose
141
Enzymatic Production of Inulooligosaccharides from Inulin
153
Vladimir Kren
165
Enzymatic Synthesis of aButylglucoside in a Biphasic
291
Enzymatic Glycosylation of Aglycones
297
Enzymatic Synthesis of Glycosides in AqueousOrganic
313
Use of pGlucosidase in the Development of Flavor
323
Index
333
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