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3T3 cells Acad affinity amino acid amino acid sequence analysis antigen assay binding Biol biological activity blot bovine IL-2 cDNA clones cDNA library cell lines cellular characterization clone coli containing culture cytotoxic encoding expression vector fibroblast genomic Gillis GM-CSF growth factor homology human IL-2 HuTNF-6 hybridized I-labeled IAP genes IFN-a IgE-binding factor IL-2 receptor Immunol induced interferon interleukin-1 isolated leukocyte lymphocytes lymphokine Lymphotoxin macrophage mature medium molecules monoclonal antibody monocytes mouse mRNA murine murine IL-2 receptor Natl peptide plasmid polypeptide probe Proc produced protein purified recombinant region residues SDS-PAGE specific stimulation supernatants synthetic T-cell tion transcription transfected tumor necrosis Urdal vitro yeast Yodoi