Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
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CHAPTER 2 Electron Microscopy of Macromolecular Assemblies
CHAPTER 3 TwoDimensional Averaging Techniques
CHAPTER 4 Multivariate Data Analysis and Classification of Images
CHAPTER 5 ThreeDimensional Reconstruction
CHAPTER 6 Interpretation of ThreeDimensional Images of Macromolecules
Appendix 1 Some Important Definitions and Theorems
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Three-Dimensional Electron Microscopy of Macromolecular Assemblies
Limited preview - 1996
3D projection 3D reconstruction 3D reference algorithm alignment amplitude analysis angle angular application atomic Baumeister Biol Boisset Carazo carbon film cell chapter Chiu classification clusters coli common lines complex components computed contrast transfer coordinate correlation cross-correlation cryo-electron cryo-electron microscopy cryo-EM map crystals data set defined defocus density map diffraction eigenvector electron crystallography electron microscopy equation experimental figure filter Fourier space Fourier transform Frank Glaeser grid GroEL Harauz Heel Hegerl hemocyanin image set iterative ligand macromolecules mask method molecular molecule negatively stained noise object obtained optical orientation parameters particles Penczek permission of Elsevier phase pixels problem protein Radermacher Radon transform random-conical range refinement represented reproduced with permission resolution result ribosomal subunit ribosome rotation sampling signal single-particle reconstruction Spahn spatial frequency specimen statistical Struct structure subsets technique term Three-dimensional tilt Ultramicroscopy variations vector visual Wagenknecht weighted back-projection Wiener filter X-ray crystallography