Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State

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Oxford University Press, Feb 2, 2006 - Science - 432 pages
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Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
 

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Contents

CHAPTER 1 Introduction
1
CHAPTER 2 Electron Microscopy of Macromolecular Assemblies
15
CHAPTER 3 TwoDimensional Averaging Techniques
71
CHAPTER 4 Multivariate Data Analysis and Classification of Images
145
CHAPTER 5 ThreeDimensional Reconstruction
193
CHAPTER 6 Interpretation of ThreeDimensional Images of Macromolecules
277
Appendix 1 Some Important Definitions and Theorems
319
Appendix 2 Profiles PointSpread Functions and Effects of Commonly Used LowPass Filters
327
Appendix 3 Bibliography of Methods
331
Appendix 4 Bibliography of Structures
337
Appendix 5 Special Journal Issues on Image Processing Techniques
343
References
345
Index
399
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