Mitochondria: A Practical ApproachV. M. Darley-Usmar, David Rickwood, M. T. Wilson Mitochondriais a unique and comprehensive guide to the key techniques used in both enzymology and molecular biology studies. Some of the protocols described in this book are well-established but, until now, have been scattered amongst enzymology manuals and biochemistry research journals. Other techniques are described in detail for the first time. |
Contents
Purity and Functional Characterization of Intact Mitochondria | 10 |
References | 16 |
References | 33 |
Copyright | |
23 other sections not shown
Common terms and phrases
acetate addition aliquots ammonium sulphate antibody assay ATPase Biol blot Ca² Ca2+ cells centrifuge cholate clones column complex I-III complex III containing cytochrome c oxidase D-loop dehydrogenase detergent digitonin Dilute Dissolve EDTA electron electrophoresis eluted enzyme activity ethanol ethidium bromide Figure filter final concentration fraction g for 20 genetic gradient homogenizer hybridization Incubate at 28°C inhibitor inner membrane isolated labelled lysine marker measured medium method mg/ml MgCl2 mitochondria mitochondrial protein mixture mtDNA mtRNA mutants NaCl NADH nitrocellulose pellet peptide phosphate buffer phospholipid plates polymerase polypeptides potassium phosphate precipitate preparation procedure protein proton purified reaction Reagents recombinants reconstitution remove replication Resuspend RNA polymerase room temperature sample saturation sodium sonication step stirring stock solution strain substrate subunits sucrose supernatant suspension Table tion tissue transcription Tris-HCl tube vitro volume wash yeast YPglu µg/ml