Mitochondria: a practical approach
Mitochondria is a unique and comprehensive guide to the key techniques used in both enzymology and molecular biology studies. Some of the protocols described in this book are well-established but, until now, have been scattered amongst enzymology manuals and biochemistry research journals. Other techniques are described in detail for the first time.
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Purity and Functional Characterization of Intact Mitochondria
ELECTRON MICROSCOPY OF MITOCHONDRIA
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absorbance acetate addition aliquots ammonium sulphate antibody assay ATPase Biochem Biol blot cells centrifuge cholate citrate synthase clones column complex complex III containing cuvette cytochrome c oxidase D-loop dehydrogenase deoxycholate detergent digitonin Dilute Dissolve EDTA electron electrophoresis elute enzyme activity ethanol ethidium bromide Figure filter filtration final concentration fraction g for 20 genetic genome gradient homogenizer incubation inhibitor inner membrane isolated labelled layer lysine marker matrix measured medium method mg/ml mitochon mitochondria mitochondrial protein mitochondrial suspension mixture mtDNA mtRNA mutants NADH nucleotides oxidized oxygen phosphate buffer phospholipid plates polymerase polypeptides potassium phosphate precipitate preparation procedure protein protein concentration proton purification reaction reagents reconstitution remove replication rotenone sample saturation sodium sonication specimen stained step stirring stock solution substrate subunits succinate sucrose supernatant Table techniques tion tissue transport Tris-HCl tube valinomycin volume wash yeast