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Action of Selenium
EXPERIMENTAL AND RESULTS
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addition alkaline ammonium hydroxide amounts of selenium analysis analytical method antinecrogenic basal medium beaker brewers bromine catalytic colorimetric concentrated nitric acid concentrated sulfuric acid condenser described determination of selenium diaminobenzidine digestion flask digestion residue dipiaselenole distilled water drying elemental selenium evaporated to dryness experiments fermentation fermentor Footnotes to Table glass glass-distilled water gram samples ground glass joint heating hydrobromic acid distillate hydrobromic acid solution liver necrosis loss of selenium MATERIALS AND METHODS methylene blue mixture of nitric nitric acid optical densities organic matter oxidation procedures perchloric acid radioactive selenious acid radioactive selenium radioactivity determination radioactivity recoveries rats reaction mixture reagent blank recoveries obtained recovery of selenium reduced removed room temperature selenious acid solution selenium added selenium content showed sodium hydroxide sodium selenite sodium sulfite standard-tapered steam bath sulfuric acid synthetic medium thioglycolic thioglycolic acid toluene torula yeast tube volume washed yeast grown