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15 minutes 48 hours 70 per cent absolute alcohol alcohol-chloroform allowed ammonia ammoniacal silver ammonium bromide astrocytes blotted blue brain stem celloidin cent alcohol cent neutral formalin cent silver nitrate cent sodium thiosulphate cerebellum cerebral changes of distilled clear and mount clearing xylol Colour Plate coronal sections cortex Counterstain coverslip cranial cresyl violet crystal violet Dehydrate differentiation distilled water dura filter paper fish hook fixation formalin formol ammonium bromide formol saline frozen sections Gieson glial fibres gliosis gold chloride gold sublimate haematoxylin Holzer Hortega's impregnation knife lesions lobe microtome mixture mordant mounting block mounting medium myelin sheaths nerve cells nervous system nitrocellulose nitrocellulose block overnight pathology Petri dish phosphomolybdic acid phosphotungstic acid-haematoxylin potassium permanganate removed Rinse in distilled silver carbonate silver nitrate skull slide sodium thiosulphate spinal cord surface tap water tartrazine technique thick Tone in 0.2 transferred vertebral arteries Wash in distilled Woelcke