Culturing Nerve Cells
Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.
The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development.
Contributors: Janet Alder, Hannelore Asmussen, Gerard Bain, Gary Banker, Robert W. Baughman, Richard P. Bunge, Ann Marie Craig, Matthew E. Cunningham, Dominique Debanne, Stephen E. Farinelli, Michael F. A. Finley, Gerald D. Fishbach, Beat H. Gä hwiler, W.-Q. Gao, Daniel J. Goldberg, Kimberly Goslin, David I. Gottlieb, Lloyd A. Greene, Mary Beth Hatten, Dennis Higgins, James E. Huettner, Kenneth A. Jones, Naomi Kleitman, Raul Krauss, Ronald M. Lindsay, Nagesh K. Mahanthappa, Carol A. Mason, Margot Mayer-Prö schel, R. Anne McKinney, Mary E. Morrison, Mark Noble, David S. Park, Paul H. Patterson, Mu-ming Poo, Richard T.Robertson, Samuel Schacher, Michael M. Segal, Carolyn L. Smith, Nacira Tabti, Scott M. Thompson, Roseann Ventimiglia, Ginger S. Withers, Patrick M. Wood, Min Yao.
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Types of Nerve Cell Cultures Their Advantages and Limitations
Primary Dissociated Cell Cultures
Transfecting Cultured Neurons
Characterizing and Studying Neuronal Cultures
Choosing the Right System
Neuronlike Cells Derived in Culture from P19 Embryonal Carcinoma
Culturing Spinal Neurons and Muscle Cells from Xenopus Embryos
Rat Hippocampal Neurons in LowDensity Culture
Rat Striatal Neurons in LowDensity SerumFree Culture
Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial
Purification and Coculture of Identified Cell Populations
Organotypic Slice Cultures of Neural Tissue
Culture of Astrocytes Oligodendrocytes and 0 2A Progenitor Cells
Tissue Culture Methods for the Study of Myelination