Rational Design and Synthesis of Endoglycosidase Inhibtors: A Case Study on Cellulases Isolated from Thermomonospora FuscaCornell University, 1991 - 446 pages |
Contents
Introduction 1 | 11 |
Exoglycosidase Inactivators | 23 |
Endoglycosidase Inhibitors | 54 |
Copyright | |
8 other sections not shown
Common terms and phrases
¹H-NMR CDCl3 50 µL aliquot a-Glucosidase by 65 acid AcO AcO active active site Add 50 µL added alcohol almond ẞ-glucosidase aromatics assays azaoligosaccharides benzyl BnO BnO BnO BnO H BnO NBn buffer to tube Carbohydr cellulase CH2OH Chem chlorohydrin chromatographed through silica CIMS concentration deionized water deprotection disaccharide epoxide epoxide 65 equiv ether evaporated to dryness exoglycosidases extracted with CH2Cl2 filtered glucose glycine glycine buffer glycosidases glycosidic glycosidic bond H₁ Háb HBF4 hexane:EtOAc hydrolysis hydroxyl Inactivation of Almond inhibitors IR(film Kinetic lysozyme m/e relative intensity methanol mixture was stirred mL of glycine mmol NaBH4 NBn BnO nucleophilic OCH2Ph OCH3 OH H OH OH OH oligosaccharides orthoester Ph2CH3P PhCHN2 phenyldiazomethane piperidine pyridine pyrrolidines reaction was stirred residue room temperature Scheme solution to 50 substrate solution synthesis TMSO vacuo yeast Yeast a-Glucosidase yield µL of substrate