PCR in NeuroscienceThe volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike.
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Contents
27 | |
Gene Synthesis by PCR | 75 |
Quantitative PCR | 103 |
Application of PCR in Mutation Detection | 161 |
Generation of Probes by PCR | 225 |
PCR in the Context of Cloning and Constructing Libraries | 253 |
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Common terms and phrases
Acad adaptor agarose gel agarose gel electrophoresis aliquot amplified DNA analysis annealing assay band BioTechniques blood cDNA synthesis centrifugation clones concentration containing cycles deletion denaturation detection digestion dilution DNA fragments DNA polymerase DNA template DNase dNTP EDTA ethanol ethidium bromide Exon extracted formamide gel electrophoresis gene genomic genomic DNA HTLV-I hybridization incubated infected isolated lane ligation lock-docking megaprimer method MgCl2 molecular molecules mRNA mtDNA mutations Natl Notſ Nucleic Acids Nucleic Acids Res nucleotides oligonucleotides PCR amplification PCR buffer PCR primers PCR products PCR reaction pellet plasmid pmol poly(A polymerase chain reaction primer Proc procedure protein protocol PTLV purified quantitative reaction mixture receptor region restriction enzyme reverse transcriptase RNA probe RNase room temperature RT-PCR samples single-stranded Southern blot specific SSCP standard strand Taq polymerase target technique tion tissue total RNA transcription Tris-HCl tube vector volume wild-type zinc finger