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active transport agents albumin albumin-bound amino acids antibodies antigens Bassingthwaighte binding Biochem Biol biological membranes biological transport Biophys blood flow blood-brain barrier brain brane capillary capillary transit carrier cell membrane cellular cholesterol clearance complex components compounds concentration corticosterone coupling debinding diffusion dilution curves distribution drug effect enzymes Equation equilibrium estimates estradiol exchange extraction facilitated diffusion fatty acids Figure flux flux-ratio free dialyzable free fraction function globulin glucose Goresky gradient human indicator dilution injection interaction intracellular ions isotope kinetics labeled ligand lipid liver measured mechanism mediated metabolism molecules myocardial Nucl organ permeability Physiol plasma protein potential progesterone propranolol protein-bound radioactivity radiolabeled radiopharmaceuticals radiotracers rate coefficients ratio receptor red cells relative renal serum solute specific steroid hormones structure studies substrate technique thyroid tion tissue tracer tracer flow transferrin translocation transport system tryptophan tumor uptake vitro vivo washout