Parthenogenesis in the Sea Urchin Lytechinus Pictus |
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Page 21
... sea water . This same filtered sea water is used throughout our experiments . Preparation of Activating Agents . The ... normal sea water . The ionophore A23187 was a gift from Dr. R.L. Hamil , Eli Lilly Co. , Indianapolis , Ind . and ...
... sea water . This same filtered sea water is used throughout our experiments . Preparation of Activating Agents . The ... normal sea water . The ionophore A23187 was a gift from Dr. R.L. Hamil , Eli Lilly Co. , Indianapolis , Ind . and ...
Page 22
... normal sea water . The total period in hypertonic sea water , including settling and centrifugation , was 15 minutes . Ionophore Activation . The ionophore solution was added to an egg suspension ( approximately 175 eggs per ml ) to a ...
... normal sea water . The total period in hypertonic sea water , including settling and centrifugation , was 15 minutes . Ionophore Activation . The ionophore solution was added to an egg suspension ( approximately 175 eggs per ml ) to a ...
Page 27
... sea water and returned to normal sea water , the cortical granules retain approximately the same appearance as at 40 minutes . These observations correlate well with the appearance of the fertilization membrane on eggs fertilized ...
... sea water and returned to normal sea water , the cortical granules retain approximately the same appearance as at 40 minutes . These observations correlate well with the appearance of the fertilization membrane on eggs fertilized ...
Contents
Development and Life 2045 | 20 |
Activation of Sea Urchin 4661 | 46 |
Conclusions and Future Investigations 6267 | 62 |
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Common terms and phrases
activating agents amino acid ammonia Arbacia artificial parthenogenesis artificially activated eggs Biol blastulae CALIFORNIA/SANTA CRUZ UNIVERSITY cell division Cell Res cleavage stages cortical granules CRUZ The University cytoplasm diploid DNA synthesis egg activation eggs exposed eggs of Lytechinus eggs suspended embryos exposure female fertilization membrane fertilized controls fertilized eggs Feulgen stained filtered sea water gastrulation genes hand centrifuged haploid haploid syndrome Hinegardner hypertonic sea water initiated ionophore larvae Ledebur-Villiger Library The University Loeb Lytechinus pictus eggs Mazia NH4OH activated normal sea water nuclear nuclei parthenogenetic parthenogenetic larvae parthenogenetic urchins Physiol Pincus plasma membrane ploidy polar body polyspermy producing pronase protease protein synthesis proteolytic enzymes resuspended SANTA CRUZ Schuel sea urchin eggs second polar body sperm Steinhardt and Epel swimming blastulae thymidine thymidine incorporation trypsin Tyler unfertilized sea urchin University Library UNIVERSITY UNIVERSITY OF CALIFORNIA UNIVERSITY OF CALIFORNIA/SANTA uptake Vacquier vitelline layer Zool