Parthenogenesis in the Sea Urchin Lytechinus Pictus |
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Page 21
... resuspended in 600 ml of NH4OH sea water . The suspension was stirred for 50 minutes at 18 ° C by a plastic paddle attached to a 30 RPM clock motor . The eggs were then allowed to settle for ten minutes , the supernatant was removed by ...
... resuspended in 600 ml of NH4OH sea water . The suspension was stirred for 50 minutes at 18 ° C by a plastic paddle attached to a 30 RPM clock motor . The eggs were then allowed to settle for ten minutes , the supernatant was removed by ...
Page 22
... resuspended in normal sea water . The total period in hypertonic sea water , including settling and centrifugation , was 15 minutes . Ionophore Activation . The ionophore solution was added to an egg suspension ( approximately 175 eggs ...
... resuspended in normal sea water . The total period in hypertonic sea water , including settling and centrifugation , was 15 minutes . Ionophore Activation . The ionophore solution was added to an egg suspension ( approximately 175 eggs ...
Page 27
... resuspended in ordinary sea water and fertilized at 10 minutes , a normal looking membrane is formed . After 20 minutes a membrane is formed with the perivitelline space greatly reduced in width . After 40 minutes membranes can no ...
... resuspended in ordinary sea water and fertilized at 10 minutes , a normal looking membrane is formed . After 20 minutes a membrane is formed with the perivitelline space greatly reduced in width . After 40 minutes membranes can no ...
Contents
Development and Life 2045 | 20 |
Activation of Sea Urchin 4661 | 46 |
Conclusions and Future Investigations 6267 | 62 |
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Common terms and phrases
activating agents amino acid ammonia Arbacia artificial parthenogenesis artificially activated eggs Biol blastulae CALIFORNIA/SANTA CRUZ UNIVERSITY cell division Cell Res cleavage stages cortical granules CRUZ The University cytoplasm diploid DNA synthesis egg activation eggs exposed eggs of Lytechinus eggs suspended embryos exposure female fertilization membrane fertilized controls fertilized eggs Feulgen stained filtered sea water gastrulation genes hand centrifuged haploid haploid syndrome Hinegardner hypertonic sea water initiated ionophore larvae Ledebur-Villiger Library The University Loeb Lytechinus pictus eggs Mazia NH4OH activated normal sea water nuclear nuclei parthenogenetic parthenogenetic larvae parthenogenetic urchins Physiol Pincus plasma membrane ploidy polar body polyspermy producing pronase protease protein synthesis proteolytic enzymes resuspended SANTA CRUZ Schuel sea urchin eggs second polar body sperm Steinhardt and Epel swimming blastulae thymidine thymidine incorporation trypsin Tyler unfertilized sea urchin University Library UNIVERSITY UNIVERSITY OF CALIFORNIA UNIVERSITY OF CALIFORNIA/SANTA uptake Vacquier vitelline layer Zool