Hormone Action, Part A, Steroid Hormones
Elsevier Science, Feb 11, 1975 - Science - 575 pages
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
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Theory of ProteinLigand Interaction
Purification of ProgesteroneBinding Plasma EDWIN MILGROM PIERRE
NISWENDER A M
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acetate acid activity added addition affinity aliquot amount analysis antibody applied assay binding Biochem Biol blank bound buffer calculated cells centrifugation Chem chromatography Clin collected column complex compound concentration constant containing counting cytoplasmic cytosol derivatives described determined developed diluted dissociation effect eluted enzyme estimated estradiol estrogen ethanol ether experiments extract final fraction glucose gradient homogenate hormone increase incubation isolated labeled material measured medium method minutes mixed mixture molecules nuclear nuclei obtained peak pellet plasma pool possible precipitate prepared present procedure progesterone protein purification radioactivity ratio reaction receptor removed sample sedimentation separation Sephadex serum sodium solution solvent specific specific activity standard step steroid steroid hormones studies sucrose supernatant suspension Table technique Teflon temperature testosterone tion tissue tube values various volume washed yield