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Protoplast Fusion and Parasexual Hybridization of Higher
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analog analysis Arabidopsis Brassica brids callus Callus Callus cell cultures cell hybrids cell lines characters chlorophyll deficient chromosome numbers chromosomes clones colonies combinations completely containing crossing culture cytoplasmic male sterility debneyi defective demonstrated determinants diploid experiments extranuclear genes fertile flower formation four Fraction I protein fragments fused fusion products genes genetic glauca Gleba growth heterozygotes higher plants hybrid cells hybrid plants inactivation inheritance initial irradiation isoenzymes isolated large subunit leaf Melchers mesophyll protoplasts method morphogenesis morphologically mutant nature Nicotiana tabacum normal nuclear nuclei observed obtained origin parasexual hybridization parental cells parental forms performed Petunia plant cells plants regenerated plasmagenes plastids plastome possessed possible Power present progeny protoplast fusion regenerated resistance restriction revealed root Schieder segregation selection sexual Shoot similar somatic cell somatic hybrids species studies Table techniques tion tissues tobacco transfer variegated vitro