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G MOREL Allocution douverture
PROBLÈMES RELATIFS A LISOLEMENT DE PROTOPLASTES PROBLEMS RELATED
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acid activity aggregates appears callus cell cultures cell division cell wall cellulase cellules changes chloroplasts chromosome Cocking concentration containing culture cytoplasm DISCUSSION effect electron enzymatically enzyme et al experiments followed formation formed fused fusion GAMBORG genetic growth haploid higher hybrid important increase incubation indicate induced infection intact isolated protoplasts leaf protoplasts leaves light mannitol material mechanical medium membrane method microscope milieu Miller mixture Nature normal nuclear nuclei observed obtained occurs origin paroi Petunia plant cells plant protoplasts plasmalemma plasts PLATE possible Power preparation present produced protein proto protoplast isolation regeneration release removed reported root showed similar solution somatic sorbitol spontaneous stages staining structure studies sucrose surface suspension synthesis Table Takebe technique tissue tobacco treatment types uptake vacuolated virus vitro wall regeneration washed