HPLC in Enzymatic Analysis

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Wiley, Apr 21, 1998 - Science - 480 pages
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The use of High Performance Liquid Chromatography (HPLC) techniquesin the study of enzymatic reactions has grown significantly sincethe publication of the first edition of this highly successfulbook: the role of enzymes in biological research has expanded; theapplication of HPLC and enzymes has extended to more disciplines;advances in separation techniques and instrumentation haveincreased the capability of HPLC; and the discovery of new enzymeshas spawned new methods of analysis.

High Performance Liquid Chromatography in Enzymatic Analysis,Second Edition addresses these developments in its coverage of therefinements of HPLC methods and their use in a wide range oflaboratory applications. It offers the same practical approachfound in the first edition, incorporates a wealth of newinformation into existing chapters, and adds new chapters to dealwith new applications, including capillary electrophoresis,forensic chemistry, microdialysis, and the polymerase chainreaction. Topics include:
* Application of HPLC to the assay of enzymatic activities
* Concepts and principles of HPLC, including the latesttechnological advances
* Concepts and principles of capillary electrophoresis (CE)
* Strategy for design of an HPLC/CE system for assay of enzymeactivity
* Preparation of enzymatic activities from tissues and singlecells
* Analysis of enzymatic activities in body fluids, includingchromatobiosis
* HPLC for the identification of new enzymatic activities
* Fundamentals of the polymerase chain reaction
* HPLC in forensics
* Survey of enzymatic activities assayed by the HPLC method,including many new categories
* Multienzyme systems, including many new examples
* HPLC in the analysis of contaminated food

"It is the ability of HPLC to accomplish separations completely andrapidly that led to its original application to problems in thelife sciences, particularly those related to purification. Ananalysis of the literature revealed that this technique was usedprimarily for the purification of small molecules, macromoleculessuch as peptides and proteins, and more recently, antibodies. Thisapplication to purification has all but dominated the use of themethod, and there has been a plethora of books, symposia, andconferences on the use of HPLC for these purposes. However, it wasonly a matter of time before others began to look beyond and toexplore the possibilities that result from the capacity to makeseparations quickly and efficiently." --from the preface to theFirst Edition

Easy to read and full of practical advice and hundreds of diagramsand examples, High Performance Liquid Chromatography in EnzymaticAnalysis, Second Edition is an invaluable resource for students,researchers, and laboratory workers in analytical chemistry andbiochemistry, molecular biology and cell biology, and for anyoneinterested in keeping up with this fast-growing field.

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About the author (1998)

EDWARD F. ROSSOMANDO, DDS, PhD, is a professor in the Department of BioStructure and Function at the University of Connecticut Health Center in Farmington and the founder and Director of the Waterborne Disease Center, a research facility for the environmental sciences. Professor Rossomando is coeditor of the book Morphogenesis and serves as a member of the editorial board of the journal Differentiation. A recognized authority in the field of chromatography and separation technologies, he has served as a member of the editorial board of the Journal of Chromatography, and he has lectured on his research at universities in the United States, Europe, the former Soviet Union, Japan, and Taiwan.

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