Light and Video MicroscopyThe purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.
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Contents
| 1 | |
| 15 | |
3 The Dependence of Image Formation on the Nature of Light | 43 |
4 BrightField Microscopy | 79 |
5 Photomicrography | 97 |
6 Methods of Generating Contrast | 111 |
7 Polarization Microscopy | 127 |
8 Interference Microscopy | 169 |
12 Various Types of Microscopes and Accessories | 219 |
13 Video and Digital Microscopy | 239 |
14 Image Processing and Analysis | 255 |
15 Laboratory Exercises | 271 |
References | 287 |
Light Microscopy A Retrospective | 327 |
A Final Exam | 331 |
A Microscopists Model of the Photon | 335 |
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Common terms and phrases
Abbe aberration amplitude analyzer angular aperture angular wave number atoms azimuth of maximal back focal plane beam birefringent bright bright-field calcite cells Chapter color compensator confocal microscope crystal dark-field differential interference contrast diffraction pattern digital camera distance electric field electrons energy extraordinary wave Figure film filter fluorescence fluorescence microscopy focal length focus front focal plane glass Huygens image formed image plane intensity interference microscope Ko¨hler illumination laser lenses light microscope linearly polarized linearly polarized light London magnification maximal transmission medium micro mirror molecules negative numerical aperture objective lens oblique illumination observed ocular optic axis optical path difference optical path length ordinary wave p-type semiconductor parallel particles phase angle photon pinhole pixel plate position prism produced propagates radiation rays reflected refractive index resolving power retardation retina rotated scanning signal slit slow axis spherical spherical aberration sub-stage condenser tion vector vibration visual wave plate wavelength Wollaston prism