Handbook of Single-Molecule Biophysics

Front Cover
Peter Hinterdorfer, Antoine van Oijen
Springer Science & Business Media, Dec 24, 2009 - Science - 626 pages

During the last decade, a number of novel biophysical methods have been developed that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at the fundamental level of sensitivity, that of a single molecule, has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them.

Editorial Advisory Board: Daniel Müller, Cheng Zhu, Claus Seidel, Xiaowei Zhuang, Thomas Schmidt, Nynke Dekker.

 

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Contents

1 SingleMolecule Fluorescent Particle Tracking
1
2 SingleMolecule Analysis of Biomembranes
19
3 SingleMolecule Imaging in Live Cells
43
4 Fluorescence Imaging at SubDiffractionLimit Resolution with Stochastic Optical Reconstruction Microscopy
95
Methods and Biological Applications
129
6 SingleMolecule Enzymology
165
7 SingleMolecule Studies of Rotary Molecular Motors
183
8 Fluorescence Correlation Spectroscopy in Living Cells
217
12 SingleMolecule Manipulation Using Optical Traps
340
13 Magnetic Tweezers for SingleMolecule Experiments
371
14 Folding of Proteins under Mechanical Force
397
15 Probing the Energy Landscape of ProteinBinding Reactions by Dynamic Force Spectroscopy
407
16 Probing Single Membrane Proteins by Atomic Force Microscopy
449
17 HighSpeed Atomic Force Microscopy
487
18 Recognition Imaging Using Atomic Force Microscopy
525
19 Atomic Force Microscopy of Protein0Protein Interactions
555

9 Precise Measurements of Diffusion in Solutionby Fluorescence Correlations Spectroscopy
243
10 SingleMolecule Studies of Nucleic Acid InteractionsUsing Nanopores
265
Generation Engineering and SingleMolecule Applications
293
20 A New Approach to Analysis of SingleMolecule ForceMeasurements
571
Extracting Informationfrom Individual Binding Events and Their Correlation
591
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