Optogenetics: Light-driven Actuators and Light-emitting Sensors in Cell BiologySophie Vriz, Takeaki Ozawa Optogenetic tools have allowed significant advances in the understanding of biological problems, particularly in the neurosciences field. Biological tools as well as optical set-ups have evolved and a wide range of probes and light-controllable modules are now available. The aim of this book is to give a flavour of illumination strategies and imaging with an overview of the different optogenetic tools and their main applications in cell biology. Based on examples covering the different aspects of cell biology, this book provides a practical approach for using light-emitting sensors and light-driven actuators. |
Contents
Fast Volumetric Imaging Using Lightsheet | 4 |
Chapter | 5 |
Superresolution Microscopy | 25 |
The Glowing Panoply of Fluorogenbased | 43 |
Optogenetic Reporters for Cell Biology | 63 |
Spatiotemporal Dynamics | 101 |
Lightdriven Actuators and Lightemitting Sensors in Cell Biology | 117 |
Quantitative Control of Kinase Activity with | 149 |
Light Control of Transcription in Cells | 169 |
Building Lightinducible Receptor Tyrosine | 181 |
Mechanotransduction and Optogenetics | 197 |
| 221 | |
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Acad actin activation allowed apoptosis Ascl1 beam biliverdin binding Biol blue light Ca2+ CALI caspases Cell Biology cell migration cellular Chem chromophore confocal confocal microscopy contractility Cry2 Cry2–Akt cytosol dimerization Dll1 domain downstream dynamics emission engineered excitation expression Figure fluorescent proteins Fluorogen-based fluorogenic fluorophores FP-based FRET function fused GECIs gene green fluorescent protein GTPases Hes1 illumination imaging inactivation induce Integrins intensity interactions irradiation with blue kinase kinetics lamellipodia laser light-induced light-sheet LSFM Lukyanov mechanotransduction Methods microscopy miniSOG modulate molecular molecules Natl neural stem cells neurons Neurosci optical optoFGFR1 optogenetic optogenetic reporters optogenetic tools oscillations oxygen PA-Akt system phosphorylation photons photosensitizers PhyB–PIF plasma membrane pMag Proc R. E. Campbell R. Y. Tsien receptor regulation RTKs signal transduction signaling pathway singlet oxygen spatial spatiotemporal specific stimulation strategy super-resolution target temporal tion tissue translocation variants wavelength zebrafish Zhang