Quantifying Interactions of Biomolecules with Inorganic Surfaces

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Springer, Dec 1, 2016 - Science - 102 pages
This thesis demonstrates the adaptation of existing techniques and principles towards enabling clean and precise measurements of biomolecules interacting with inorganic surfaces. In particular, it includes real-time measurement of serum proteins interacting with engineered nanomaterial. Making meaningful and unambiguous measurements has been an evolving problem in the field of biology and its various allied domains, primarily due to the complex nature of experiments and the large number of possible interferants. The subsequent quantification of interactions between biomolecules and inorganic surfaces solves pressing problems in the rapidly developing fields of lipidomics and nanomedicine.
 

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Contents

1 Introduction
1
2 Methods
7
3 Quantifying Interactions Between Water and Surfaces
27
4 Quantifying Interactions Between Lipids and Surfaces
41
5 Quantifying Interactions Between Serum Proteins and Gold Nanoparticles
68
6 Conclusion
91
SPR Sensorgrams for Interactions of 3 Serum Proteins with Gold Nanoparticles
95
SPR Sensorgrams for Interactions of PEG with Chemically Active Chip Surface
99
Curriculum Vitae
100
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About the author (2016)

Abhijeet Patra obtained his bachelors degree in Electrical and Electronics Engineering from the National Institute of Technology, Tiruchchirappalli, one of the premier institutes in India in 2011. Upon receiving the prestigious NGS scholarship to pursue doctoral studies at National University of Singapore, he joined the research group of Prof T Venkatesan, pioneer of pulsed laser deposition and, after an exciting 4 years, graduated with a PhD in 2015. His research interests are in understanding how inorganic assemblies interact with the biological world, with a sharp focus on translating the science into biomedical applications.