Nanoscale Imaging and Characterisation of Amyloid-β

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Springer, Jul 5, 2016 - Science - 149 pages
This thesis presents a method for reliably and robustly producing samples of amyloid-β (Aβ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide’s nanomechanical, elastic, thermal or spectroscopical properties.
Amyloid-β (Aβ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of Aβ with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.
 

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Contents

1 Introduction
1
2 Theoretical Concepts of Scanning Probe Microscopy and Dynamic Light Scattering and Their Relation to the Study of Peptide Nanostructures
7
3 Alzheimers Disease and the Aggregation of Amyloid β
31
4 Experimental Methodology
53
5 Substrate Development of the Imaging of Amyloid Proteins with SPM Methods
73
6 Scanning Probe Microscopy Methods of Imaging Amyloid Peptides During the Aggregation Process
87
42
107
8 The Application of Biophysical Techniques to the Study of the Inhibition of Aggregation of Aβ Using PINPs Liposomes
121
9 Conclusion and Future Perspectives
139
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