Molecular Fluorescence: Principles and Applications

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John Wiley & Sons, May 29, 2012 - Science - 569 pages
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This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments.

The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips.

For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.

 

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Contents

Absorption of Ultraviolet Visible and NearInfrared Radiation
33
Characteristics of Fluorescence Emission
53
Bibliography
74
4
93
5
106
5
120
6
139
10
156
SteadyState Spectrofluorometry
265
A Elimination of Polarization Effects in the Measurement
281
MultiExponential Decays
290
Bibliography
323
Bibliography
346
Evaluation of Local Physical Parameters by Means
379
Bibliography
404
6
413

Emission Anisotropy
181
Case of Motionless Molecules with Random Orientation
191
Effect of Rotational Motion
199
8
213
6
227
Autofluorescence and Fluorescence Labeling in Biology and Medicine
479
Bibliography
502
Bibliography
518
Epilogue
551
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About the author (2012)

Bernard Valeur received his engineering diploma from the ï¿1⁄2cole Supï¿1⁄2rieure de Physique et de Chimie Industrielles de Paris (E.S.P.C.I.) and his PhD degree from the Universitï¿1⁄2 Pierre-et-Marie-Curie (Paris, France), followed by postdoctoral research at the University of Illinois at Urbana-Champaign (USA). After being an associate professor at E.S.P.C.I, he became full professor of physical chemistry at the Conservatoire National des Arts et Mï¿1⁄2tiers (Paris) in 1979, where he is emeritus professor since 2008. Professor Valeur is a member of the laboratory Photophysique et Photochimie Supramolï¿1⁄2culaires et Macromolï¿1⁄2culaires at the ï¿1⁄2cole Normale Supï¿1⁄2rieure de Cachan since 1996. From 1995 to 2000 he served as an elected member of the French Comitï¿1⁄2 National de la Recherche Scientifique. He is the author of over 170 articles or book chapters, five books, and the editor of one book. In addition, he is a member of several editorial boards.


Mï¿1⁄2rio Nuno Berberan-Santos graduated in chemical engineering from Instituto Superior Tï¿1⁄2cnico (IST, Technical University of Lisbon, Portugal). After a brief stay at the National Research Council of Canada (Ottawa), he received his PhD in chemistry from IST in 1989. He was a post-doctoral fellow with Bernard Valeur at Conservatoire National des Arts et Mï¿1⁄2tiers (Paris, France), and at Laboratoire pour l'Utilisation du Rayonnement Electromagnï¿1⁄2tique (Univ. Paris-Sud, Orsay, France). He is full professor of Physical Chemistry at IST, and was invited full professor at the ï¿1⁄2cole Normale Supï¿1⁄2rieure de Cachan (France). He is a member of several editorial advisory boards and is president of the Portuguese Chemical Society (2010-2012). He has authored over 180 publications, including 150 papers in scientific journals, several book chapters, and was the editor of one book.

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