Detection of Non-Amplified Genomic DNA
Giuseppe Spoto, Roberto Corradini
Springer Science & Business Media, Jul 6, 2012 - Science - 318 pages
This book offers an overview of state-of-the-art in non amplified DNA detection methods and provides chemists, biochemists, biotechnologists and material scientists with an introduction to these methods. In fact all these fields have dedicated resources to the problem of nucleic acid detection, each contributing with their own specific methods and concepts. This book will explain the basic principles of the different non amplified DNA detection methods available, highlighting their respective advantages and limitations. Non-amplified DNA detection can be achieved by adopting different techniques. Such techniques have allowed the commercialization of innovative platforms for DNA detection that are expected to break into the DNA diagnostics market. The enhanced sensitivity required for the detection of non amplified genomic DNA has prompted new strategies that can achieve ultrasensitivity by combining specific materials with specific detection tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and electrochemical detection tools are among the most widely investigated to analyze non amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics are discussed by an outstanding set of authors with proven experience in this field.
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allergenic Anal analytical applications approach assay AuNPs binding Bioelectron biological Biosens biosensor biotinylated bovine cancer cells Chem chemical complementary complex conjugated polymers Corradini denaturation detection of DNA developed diagnostics DNA detection DNA hybridization DNA probes DNA sequence DNA targets duplex electrochemical electrode enhanced enzymatic enzyme fetal fluorescence fluorophore fragments gene expression gold nanoparticles human genome immobilized interactions label-free labeled locked nucleic acids Marchelli metal methods microarrays microRNAs mismatch molecular molecules morpholino multiplex mutations nanomaterials nanoparticles nanopore nanostructures nanowire non-amplified genomic DNA nucleotide obtained oligonucleotide optical peptide nucleic acid piezoelectric plasmon resonance imaging platform polymerase protein quantitative quantum dots Raman reaction reported sample sensitivity signal amplification single single nucleotide polymorphisms single-molecule solution specific Spoto SPRI ssDNA strand strategy streptavidin structure surface plasmon resonance synthesis target DNA target sequence techniques thermal thiolated unamplified Wang Zhang